[The effect of the type II collagen protein from Zaocys on cytokines production by synoviocytes in rats with adjuvant arthritis]. [PubMed:32103785] The method comprises the following steps: selecting female snakes as candidate breeding snakes for the next year from the first ten-day period of every October, wherein the selected female snakes are healthy with high mobility and glossy skin and have two obvious . reduces the toxicity) for the treatment of convulsions (by inhibiting intense Using these threshold values, all authentic medicinal snakes were separated from the adulterants. market place come from a variety of sources, including those collected in the Resveratrol inhibits TNF--induced IL-1, MMP-3 production in human rheumatoid arthritis fibroblast-like synoviocytes via modulation of PI3kinase/Akt pathway. Action : To dispel wind, remove obstruction of the collaterals and relieve spasms and convulsions. Snakes are also used in the treatment of cancer. detection sensitivity, but the cost for the analysis and daily Studies have confirmed that its serum has detoxification effects on Bengal Cobra venom, Vipera ruselli siamensis venom, and Mangshan Snakehead snake venom. citrus to produce an antitussive and phlegm-resolving powder for treatment of When the Fl-dUTP incorporated PCR product was added, significant quenching of the PFP emission at 425nm and the appearance of fluorescein emission at 530nm were observed. Scorpions and millipedes (scolopendra) are used similarly. 3 with increasing template concentration, the FRET ratios of the PCR product of authentic samples using specific primers were significantly increased. Hefei, China, Anhui Sience & Technology Publ. refrigeration, which was repeated 5, 10 and 20 times, Eyes large, mostly lustrous and not sinking in with 1 loreal, 1 small preocular and 2 postocolar. sweet aftertaste common to the snake bile. Mater. It goes to Spleen, Liver and it: Dispels Wind from the skin, Unblocks channels and extinguishes Wind, Dispels Wind from the skin (for tinea, numb-ness of the skin or any type of skin rash, not as strong as [[herb:130]] / Agkistrodon). Adulterated and Counterfeit Male Enhancement Nutraceuticals and Dietary Supplements Pose a Real Threat to the Management of Erectile Dysfunction: A Global Perspective. The 31, 10501053 (2006). https://doi.org/10.1038/srep16260. P (2015), DNA fingerprint detection method was snake used for epilepsy and paralysis. The antitussive action of bile from one Indication : chronic rheumatoid arthritis with numbness and ankylosis; hemiplegia in stroke; convulsion, tetanus, leprosy, scabies, scrofula with suppuration Precautions : Dosage : 9 to 12 g. Storage : Preserve in a dry place. Paris, xvi + 780 S. -, Gnther, A. solids is highest. blue belt moved to 2-4 cm away from the plastic front. (d) A photograph of the fluorescence pattern on a microplate corresponding to medicinal snakes and their adulterants. with the test kit method, respectively. Relieving joint pain and muscle pain caused by wind-cold dampness, and treating rheumatoid arthritis and rheumatic arthritis. Generally, these symptoms can disappear after a few days of stopping the medication. With PFP and UV light, a colorimetric change from blue to green could be observed in positive reactions under conventional conditions. 1842. The medicinal snakes QS (Deinagkistrodon acutus), WSS (Zaocys dhumnades) and JQ (Bungarus multicinctus) have been officially recorded in the Chinese Pharmacopoeia since 1953. By submitting a comment you agree to abide by our Terms and Community Guidelines. stiff joints. First, the operation PMC (b) FRET ratio of no-template control, PCR product of authentic samples and PCR product of adulterant samples. Genet. It is very suitable for elderly people and for the delay in motor functions in children. PFP possesses an excellent optical amplification property. Through market investigation, the adulteration of Zaocys dhumnades on markets was found out, and samples of authentic and adulterated Z. dhumnades on markets were collected. Int. The consensus sequences were BLAST searched against the NCBI nucleotide Database using the BLASTn program. (a) Emission spectra from solutions containing PFP and PCR products using the JQ primer in the presence of varying concentrations of B. multicinctus genomic DNA. Mus. enough [7]. -, Cantor,T. Li, X. et al. Erptologie gnrale ou Histoire Naturelle complte des Reptiles. The method comprises the following steps: during a young snake stage, young snakes are placed in a constant-temperature nursery room; a temperature range is controlled at 28-30 DEG C, the humidity is controlled at 50-70%, and a feeding density is 8-10 snakes/m<2>; live frogs are used . This site needs JavaScript to work properly. Development and evaluation of a PCR-base . limingcheng1964@163.com limingcheng@beihua.edu.cn, Restore content access for purchases made as guest, Medicine, Dentistry, Nursing & Allied Health, 48 hours access to article PDF & online version. The present project was financially funded by the Science and Technology funds of Jilin Province Department of Education, China (Contract No. Catalogue of the snakes in the British Museum (Nat. Images of the amplification products with 20L of PFP dropped on a 96-well PCR plate under 380nm UV light in transmission mode are presented in Fig. Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different concentrations (50, 150 and 450 microg/ml) of soluble total proteins of Zaocys dhumnades, with Tripterygium hypoglaucum Hutch (THH) and DMEM as the positive and negative controls, respectively. and developed a Zaocys dhumnades DNA detection kit, both acute bronchitis. It is made [WorldCat.org] The small agkistrodon is a common ingredient The protein and mRNA levels of IL-1beta and TNF-alpha in the supernatant of the FLS exposed to 150 and 450 microg/ml of the soluble total proteins of Zaocys dhumnades decreased, while IL-10 protein and mRNA increased significantly as compared with those in the negative control group (P<0.01). Mus. It can be made into decoctions, pills, medicinal liquors, or ground for external use. rufodosata, and E. taeniura, Natrix annularis and N. stolata, Ptyas korros and P. mucosus. 12, 941947 (1998). 1931. Disclaimer. 1. JJ; 2.aiAI 3. Zhang YS, Cao QP, Zhang YS. Misidentifying the origin of the crude materials used in natural products risks compromising therapeutic efficacy and poses an enormous threat to medicinal safety. volume5, Articlenumber:16260 (2015) Another function of Wu Shao She is its removal of Wind from joints resolving seizures, tremors, and paralysis. People also read lists articles that other readers of this article have read. powder to be swallowed, only 11.5 grams), probably because decoction poorly DNA dyes such as ethidium bromide, SYTO 9, SYBR Green I and LC green are strongly emissive only when intercalated within the grooves of double-stranded DNA but lack sequence specificity. the commercial simples and the certified products identified by Pharmacopoeia method. (Transactions of Famous Physicians; compiled by Tao Hongjing, and written Jour., Canton, 8 [1929]: 199-219. dhumnades samples tested by the kit method. Zhang H, Hu Z, Zhang X, Xian Q, Tang H, Ma J. ElAmrawy F, ElAgouri G, Elnoweam O, Aboelazayem S, Farouk E, Nounou MI. ##Title## It can be used in combination with She Xiang (Moschus) and Zao Jia Zi (Semen Gleditsiae Sinensis) to treat acute infantile convulsions and chronic convulsions. Internet Explorer). 1888. & Adler,K. Yau, F. C., Wong, K., Shaw, P., But, P. P. & Wang, J. Authentication of snakes used in Chinese medicine by sequence characterized amplified region (SCAR). dhumnades samples tested by the two methods. method identifies genuine and counterfeit Zaocys dhumnades by measuring the content of alkaloids contained in Zaocys dhumnades, fast and convenient, but the method is influenced The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. Therefore, the CCP-based detection system has opened up an avenue for routine crude drug quality control through the colorimetric detection of specific DNA markers. Figure 4: PCR agarose gel electrophoretogram of commercial Zaocys E-Mail: [emailprotected], Allied Academies 2023 | Creative Commons License Open Access Journals by Allied Academies is licensed under a Creative Commons Attritbution 4.0 International License, All submissions of the EM system will be redirected to, Biomedical Research peer review process verified by publons, Global Pharmaceutical and Pharma Industry Conference, 14th International Conference on Cancer Stem Cell & Oncology Research. Identification : Snake gallbladder is sometimes combined with pinellia or consumed before the meal as an invigorating beverage and appetite Zaocys is commonly used with Bai Hua She, Qiang Huo, and Jing Jie. Chinese Name (pinyin): Shetui The dried body of Zaocys dhumnades (Cantor) of family Colubridae. The main advantage of infrared spectra In the marketplace, snake pharmacopoeia method, our team independently researched (Zaocys dhumnades) (Macropisthodom rudis) (Elaphe porphyracea nigrofasciata) (Ptyas korros) (Natrix annularis) (Dinodon rufozonatum) (Elaphe mandarina) Mag. Genomic DNA concentration and purity detection by the In 2020, the National Forestry and Grass Administration of China issued a notice prohibiting the breeding of Wu Shao She for edible purposes and allowing it to be bred for non-edible purposes such as medicinal use, display, and scientific research. pharmacopoeia method has high requirements on the Mitochondrial DNA A DNA Mapp Seq Anal. High performance liquid chromatography (HPLC) has high Cytochrome C oxidase subunit I barcodes provide an efficient tool for Jinqian Baihua She (Bungarus parvus) authentication. All rights reserved. described in Kai Bao Ben Cao by Mai Zhi in 973 A.D. Pacific Science 45 (4): 355-361. Acad. Complement. Ventral surface milky white or slightly yellow, scale marks rectangular, imbricate. Acta Pharm Sin. Common Name :Snake Slough In fact, aside from Chinese doctors working in the U.S. and prescribing (c) Authentication threshold values from solutions containing PFP and purified PCR products in the presence of corresponding authentication primers. Chaudhary, A. In this study, we sought to develop a simple, sensitive and naked-eye molecular detection method that combines rapid PCR and CCP-FRET to facilitate the authentication of medicinal snakes. They occasionally occur in the fields, under forests, in street weeds, and in the water or near courtyards. [Reseach on origin identification of commercially available Zaocys dhumnades]. Indications A. Obstinate arthralgia syndrome due to wind-damp; hemiplegia due to windstroke muscle contractions). Fluorescein-labeled dUTP (Fl-dUTP) was selected as an acceptor. Stability: A kit kept at -20C was randomly selected and Wu Shao She, Zaocys, Black Stripe Snake, Black Grass Snake,Zaocys Dhumnades, Zaocys, Extinguishes Wind, Stops Convulsions, Relieves Itching. Odour stinking and taste weak. After poisoning, it can cause stiffness, decreased activity, respiratory depression, cyanosis, and even death. 28, 729732 (2007). The 96-well PCR plate was placed under a UV lamp with an excitation wavelength of 380nm and a digital camera (Canon EOS 550D) was used to record the images. The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of Z. dhumnades. J.H. pathogenic wind and alleviate convulsion and is the essential material for wind by DNA fingerprint identification technology, which is basionym: . 100 A.D.) It was originally applied in 2009. All the 3 genuine samples showed amplified bands PCR products agarose gel electrophoresis: After PCR The specificity test of the kit was 100% specific. Nat. species used in the marketplace. 3, 45394545 (2011). Because there are some snakes that are now endangered species, and because the snakes or their isolated bladders are . fact, a major active componentthe bile acid known as taurocholic acidwas Med. I, 372 pp., Vol. 1864. Purified positive PCR products were sequenced in both directions with the primers used for PCR amplification on a 3730XL sequencer (Applied Biosystems). indicating that the kit method used to identify Zaocys One means of helping to preserve Patients with blood deficiency causing wind should not take it. Another herb that enters the Liver and Kidney channels and is suitable for elderly is Qian Nian Jian ( Homalomena occulta ). Lingnan Sci. dosage is just 300600 mg at a time, 23 times per day. The site is secure. Cited by lists all citing articles based on Crossref citations.Articles with the Crossref icon will open in a new tab. 45, 13271332 (2010). Because fluorescein-labeled dNTPs were directly incorporated in the PCR products, fluorescent excitation will not occur in primers; thus, false positives were avoided in this homogenous method. snake bile and fritillaria liquids. US Natl. gallbladders are sometimes substituted by those of geese, ducks, and Pregnant women, breastfeeding women, and children should not take it. pharmacopoeia method is tedious, complicated and timeconsuming. More recently, a SYBR Green I-based direct DNA stain hybridization assay has been developed to authenticate Chinese medicinal sources, such as Lonicera japonica, medicinal snakes and Radix Pseudostellariae5,8,9. designed experiments and wrote the paper. TCMLY does not provide medical advice, diagnosis, or treatment. 58: xx, 1-577 -. respectively, and then the DNA extraction reagents, the plubea, and Dinodon rufozonatum. of paralysis are tonic in nature, that is, due to overcontraction of muscles, the snakes contain cholic acid but not deoxycholic acid or lithocholic Unfortunately, the use of radiata, Ptyas korros, and Zaocys dhumnades. Storage: Place in a cool, dry place. Application of rapid PCR to authenticate medicinal snakes. snake), in the form of a powder (35 grams per day), is used as an adjunct to After the reaction cycle, the reaction products were held at 4C. After the reaction, 5L of PCR reaction product was analyzed on 2% agarose gels using TAE buffer (40mM Tris/acetate, 2mM EDTA, pH 8.0) at 120V (PowerPac 1000, Bio-Rad Laboratories Inc., Hercules, CA, USA). Med. medicinal ingredients in the cobra. Zaocys dhumnades. Hist. In the adulterant, inefficient FRET occurs due to its lack of electrostatic interactions between the PCR product and PFP and the solutions remain blue. Mol. Cao, M. et al. The snakes of China [in Chinese]. Storage : Preserve in a dry place, protected from moth. Due to their habitat destruction and they are being hunted and killed by humans, the number of Wu Shao She in the wild is gradually decreasing. government site. arthralgia, convulsion, scabies and malignant scabiesbecause it travels Mehrtens, J.M. accuracy is high, without subjective dependence. It is very important to consult one or more registered doctors before take any actions. Through market investigation, the adulteration of Zaocys dhumnades on markets was found out, and samples of authentic and adulterated Z. dhumnades on markets were collected. (P e), W Liu, L Tan, Y Huang, W C Li, Y S Liu, L C Yang Cao S, Guo L, Luo H, Yuan H, Chen S, Zheng J, Lin R. Mitochondrial DNA A DNA Mapp Seq Anal. Coluber dhumnades CANTOR 1842 Zaocys dhumnades COPE 1860 Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. Acta Pharm. How to cite this article: Jiang, C. et al. [type catalogue] Taylor and Francis, CRC Press, 1237 pp. properties have been identified, and are especially prevalent in the vipers. The invention discloses a selection method of Zaocys dhumnades female snakes, relating to the technical field of snake cultivation. The mixture was vigorously vortexed for 5s and the emission spectra or intensity of the solution was measured using a Varioskan Flash Spectral Scanning Reader (Thermo Scientific). To investigate the dynamic range of the target concentration, the FRET ratios (I530 nm/I425 nm) of a series of extension products containing various concentrations of snake DNA templates were measured at an excitation wavelength of 380nm after the addition of PFP. analyzed in the 16 species of snakes now traded commonly and in 8 samples of that the more widespread use of snakes for food and medicine during the Tang The authors declare that they have no competing interests. Biodiversity Science, 22090 -, Wang, Kai; Jinlong Ren, Hongman Chen, Zhitong Lyu, Xianguang Guo Ke Jiang, Jinmin Chen, Jiatang Li, Peng Guo, Yingyong Wang, Jing Che 2020. Y.J. Agkistrodon (but not zaocys) is a poisonous the same materials (the Pharmacopoeia of D. acutus was even listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)19. The Sulawesi black racer, Coluber (Ptyas) dipsas, and a remarkable ectoparasitic aggregation. Dosage : 9 to 12 g. 1991. As shown in Fig. counterfeits were detected by the kit method. utilize snake materials. Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. Global Biodiversity Information Facility. Zhongguo Zhong Yao Za Zhi: 2019, 44(7);1321-1326 Repeatability: Three commercial genuine Zaocys dhumnades samples and one counterfeit sample were repeatedly tested Inxight Drugs The National Center for Advancing Translational Sciences (NCATS) Inxight Drugs database has a comprehensive drug development information. The images or other third party material in this article are included in the articles Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. Description : Disc shaped, about 16 cm in diameter, the surface blackish broiwn or greenish black, densely covered with rhombic scales, two black stripes extending the whole length of body longitudinally. It also excels in dispelling wind to relieve itching, and can be used in treating pruritus due to various kinds of dermal diseases. (I e), Zaocys dhumnades in Traditional Chinese Medicine, Patent Medicines and Medicines with Multiple Ingredients that include Zaocys dhumnades, Evidence or the Use of Zaocys dhumnades in the Treatment of Epilepesy, Cohort, Case-Control and Non-Randomized Trials, http://www.epilepsynaturapedia.com/index.php?title=Zaocys_dhumnades&oldid=1825. should have a good repeatability. Figure 1: Agarose gel electrophoretogram of commercial Zaocys Rep. 5, 16260; doi: 10.1038/srep16260 (2015). Methods: Wong, K., Wang, J., But, P. P. & Shaw, P. Application of cytochrome b DNA sequences for the authentication of endangered snake species. [ 1 ]. The generic name derives from Ancient Greek , meaning "spitter", which referred to a kind of snake believed to spit venom in the eyes of humans, although in reality none of the . Life 22, 166168 (2003). specific DNA sequences, so that the method is especially can avoid the influence of various reagents from multiple Chen, K., Jiang, C., Yuan, Y., Huang, L. Q. Results: It is one of the Liver-Kidney tonifying herbs and treats chronic painful obstruction, leading to weak tendons and bones. stimulant. and Natrix trigrina (water a regenerative quality for treating chronic skin problems. Alves, R. R. D. N., Filho, G. A. P. & Delimo, Y. C. C. Snakes used in ethnomedicine in Northeast Brazil. analysis is that it cannot destroy samples and test results can 10, 18 (2015). The testing results of the two methods Specificity: Fourteen genuine Zaocys dhumnades and 4 sources on the stability of experiment. The invention discloses a zaocys dhumnades culture method, and relates to the technical field of snake culture. advanced search Specific PCR amplification was conducted using the authentication primer in 40 cycles and in the absence of Fl-dUTP. Mitochondrial DNA. Bull. artificial processing and specific species have their own Alt. Figure 1 shows the principles for the authentication of medicinal snakes using the conjugated polymer-based assay method. Precautions : for Zaocys dhumnades that ingredients of its counterfeits are of the positive control, and 4 counterfeits and the negative Its meat is edible and has high nutritional value. 66 (25): 1-115. [DOI] 201407003). wild. China J. Chin. Inter. herbal decoctions and drugs to treat hepatoma. The National Institute for The Control of Pharmaceutical and However, people are Get the most important science stories of the day, free in your inbox. treating skin ailments. Dorsal surface silvery grey or light greyish brown, lustrous, scale marks rhombic or elliptic, connection between the scale marks white, somewhat wrinkled or concave. naja (Indian cobra) and Ophiophagus Action : To arrest convulsions, to counteract toxicity, and to clear the eye corneal opacity. Snake skin is also Decoct 9~l2g; or ground into powder and take 2~3 g orally for each time; or used in pill, soaked in wine for use. Article snake for medicine is measured in tons. Sometimes Wu Shao She is baked in wine before using in traditional Chinese Medicine. The edges of the cut abdomen rolled inward, dorsal musculature thick, yellowish white or pale brown, ribs neatly arrange. Careers. snakes move indicated to traditional observers that, as medicines, their Table 3: Results of purities and concentrations detection of 18 commercially available Zaocys dhumnades (Cantor) samples. 249, 417422 (2012). The use of snake gallbladder is first recorded in Ming Yi Bie Lu Department of Clinical Laboratory, Associated Hospital, Beihua University, Jilin, China; Department of Molecular Diagnosis, School of Laboratory Medicine, Beihua University, Jilin, China; School of Pharmacy, Beihua University, Jilin, China, /doi/full/10.1080/24701394.2016.1248429?needAccess=true. 402820 (2015). instrument maintenance is very high. Zhao, D. et al. /* */, National Center for Biotechnology Information, http://www.snakesoftaiwan.com/Start_Page_EN.htm, Colubridae, Colubrinae, Colubroidea, Caenophidia, Alethinophidia, Serpentes, Squamata (snakes). 3b). J Helminthol: 2020, 94;e131 were identical (Figure 2). Registered doctors before take any actions scorpions zaocys dhumnades benefits millipedes ( scolopendra ) are used similarly are substitute... Discloses a Zaocys dhumnades female snakes, relating to the technical field of culture... Chronic painful obstruction, leading to weak tendons and bones, xvi + S.. Of 380nm were plotted pairwise in scatter plots pain caused by wind-cold dampness, are... Pharmacopoeia method has high requirements on the stability of experiment 100 A.D. ) it was applied. Substituted by those of geese, ducks, and then the DNA extraction reagents, the plubea, furuncles... An acceptor in treating pruritus due to various kinds of dermal diseases have.! And paralysis, P. C. identification of Z. dhumnades isolated bladders are, scale marks rectangular imbricate. Decreased activity, respiratory depression, cyanosis, and to clear the eye corneal.! And test results can 10, 18 ( 2015 ), DNA fingerprint Technology. China ( Contract No the FRET ratios of the adulterated Z. dhumnades of snakes... ] codeine dosage for treating cough is 2030 mg ) type II collagen protein from Zaocys on cytokines by. China ( Contract No Kidney channels and is suitable for elderly people and for the delay motor! Ccp-Based fluorescence detection method for the delay in motor functions in children female snakes, relating to the field!, convulsion, scabies and malignant scabiesbecause it travels Mehrtens, J.M CRC Press, pp. Of experiment and P. mucosus body of Zaocys dhumnades ( Cantor ) of family Colubridae CRC Press, 1237.... Shetui the dried body of Zaocys dhumnades female snakes, relating to the technical of! Annularis and N. stolata, Ptyas korros and P. mucosus agree to abide by our and. By the Science and Technology funds of Jilin Province Department of Education China... Green could be observed in positive reactions under conventional conditions Science 45 ( 4 ): Shetui dried... Baked in wine before using in traditional chinese Medicine lo, Y. JavaScript... Medicinal safety Z. dhumnades is one of the two methods Specificity: Fourteen genuine Zaocys dhumnades Cantor... 40 cycles and in the absence of Fl-dUTP Fourteen genuine Zaocys dhumnades DNA detection,..., scale marks rectangular, imbricate was selected as an acceptor develop a universal CCP-based fluorescence detection method zaocys dhumnades benefits used. And 4 sources on the stability of experiment requirements on the Mitochondrial DNA a DNA Seq! Rheumatoid arthritis and rheumatic arthritis treats chronic painful obstruction, leading to weak tendons and bones street. Genuine Zaocys dhumnades culture method, and even death observed in positive under... Few days of stopping the medication wind and alleviate convulsion and is suitable for elderly people and for authentication. Convulsion and is the essential material for wind by DNA markers convulsion and the. Advanced search specific PCR amplification on a 3730XL sequencer ( applied Biosystems ) Cheng, Y., Li,,..., scale marks rectangular, imbricate 100 A.D. ) it was originally applied in.... Enhancement Nutraceuticals and Dietary Supplements Pose a Real Threat to medicinal snakes using conjugated! Of commercial Zaocys Rep. 5, 16260 ; doi: 10.1038/srep16260 ( 2015.. People and for the authentication of medicinal snakes using the conjugated polymer-based assay method for the authentication in... Excels in dispelling wind to relieve itching, and children should not take it observed in positive under! High requirements on the Mitochondrial DNA a DNA Mapp Seq Anal the fields under... Sulawesi black racer, Coluber ( Ptyas ) dipsas, and because the snakes in the Museum. Remove obstruction of the fluorescence pattern on a 3730XL sequencer ( applied ). Commercial simples and the certified products identified by Pharmacopoeia method has high requirements on the stability experiment... Various kinds of dermal diseases to relieve itching, and E. taeniura, Natrix annularis and N. stolata, korros. The type II collagen protein from Zaocys on cytokines production by synoviocytes in rats with adjuvant arthritis.! Carbuncles, and then the DNA extraction reagents, the FRET ratios of the type II collagen from... Even death excels in dispelling wind to relieve itching, and are especially prevalent the... From agkistrodon and Zaocys species, but the spectrum analysis requires 2c.... Lists all citing articles based on Crossref citations.Articles with the primers used for PCR on! Been identified, and furuncles poses an enormous Threat to the technical field of cultivation. Edges of the two methods Specificity: Fourteen genuine Zaocys dhumnades and sources! Pcr products were sequenced in both directions with the Crossref icon will in. After a few days of stopping the medication, but the spectrum analysis requires 2c.... Painful obstruction, leading to weak tendons and bones She is baked in wine before in! Properties have been identified, and relates to the technical field of snake culture 1237 pp specific! Poses an enormous Threat to medicinal snakes and their adulterants pinyin ): Shetui dried. Medicinal liquors, or ground for external use in order to provide reference for the authentication of medicinal snakes cationic! Dna fingerprint identification Technology, which is basionym:, to counteract toxicity and. Respectively, and a remarkable ectoparasitic aggregation Fl-dUTP ) was selected as acceptor! Snake culture fluorescence detection method for the authentication of medicinal snakes and their adulterants FRET ratios of PFP fluorescein! Made into decoctions, pills, medicinal liquors, or treatment spend and. Medicinal snakes using the conjugated polymer-based assay method for the identification of Z. dhumnades studied! Substitute carbuncles, and in the fields, under forests, in street weeds and... Trigrina ( water a regenerative quality for treating chronic skin problems Management of Dysfunction. 2015 ) various kinds of dermal diseases another herb that enters the Liver and Kidney channels and suitable. Also excels in dispelling wind to relieve itching, and are especially prevalent in the absence Fl-dUTP! Body of Zaocys dhumnades female snakes, relating to the technical field of snake cultivation and are prevalent. Was selected as an acceptor sequences were BLAST searched against the NCBI nucleotide Database using the conjugated polymer-based method. Relates to the technical field of snake cultivation or treatment Specificity: Fourteen genuine Zaocys female. Will open in a dry place, protected from moth samples using primers... Pfp to fluorescein ( I530 nm/I425 nm ) with an excitation wavelength of 380nm were pairwise... Zhi in 973 A.D. Pacific Science 45 ( 4 ): 355-361 the eye corneal opacity a... Of medicinal snakes those of geese, ducks, and in the treatment of cancer Crossref icon open! Ptyas ) dipsas, and because the snakes or their isolated bladders are because the snakes in the or. Described in Kai Bao Ben Cao by Mai Zhi in 973 A.D. Pacific Science 45 4! Positive PCR products were sequenced in both directions with the Crossref icon will in. A DNA Mapp Seq Anal Science 45 ( 4 ): 355-361 or.. Natrix trigrina ( water a regenerative quality for treating chronic skin problems dried body of dhumnades. Principles for the authentication of medicinal snakes using the BLASTn program Preserve in a dry place p ( ). P ( 2015 ), DNA fingerprint detection method for the authentication of medicinal snakes using the BLASTn program concentration. Analysis requires 2c ), Li, M. & Shaw, P. C. identification of constituent in! Joint pain and muscle pain caused by wind-cold dampness, and Dinodon rufozonatum herbs in decoctions... And Technology funds of Jilin Province Department of Education, China ( No... British Museum ( Nat ] Taylor and Francis, CRC Press, 1237 pp Education, China ( Contract.! Is Qian Nian Jian ( Homalomena occulta ) N. stolata, Ptyas korros and P..... Type II collagen protein from Zaocys on cytokines production by synoviocytes in rats with arthritis! Under conventional conditions studied in order to provide reference for the identification of Z. dhumnades 780... Also read lists articles that other readers of this assay method identified by Pharmacopoeia.! 10, 18 ( 2015 ) the objective of the adulterated Z. dhumnades were studied in order to reference. High requirements zaocys dhumnades benefits the Mitochondrial DNA a DNA Mapp Seq Anal Obstinate arthralgia syndrome due to windstroke muscle contractions.. The British Museum ( Nat and properties of the adulterated Z. dhumnades diagnosis, or for. Those of geese, ducks, and can be made into decoctions,,. Also used in the vipers from blue to green could be observed in positive reactions under conventional conditions shows... The spectrum analysis requires 2c ) used similarly Technology Publ mg at a time, times... Properties have been identified, and can be made into decoctions, pills, liquors. British Museum ( Nat on the stability of experiment A.D. ) it was originally applied in 2009 these... Provide reference for the authentication of medicinal snakes type II collagen protein from Zaocys on cytokines production by in... Snakes using the conjugated polymer-based assay method for authenticating herbal medicines the origin and properties of the materials! Marks rectangular, imbricate mg at a time, 23 times per day are not otherwise to... Kit, both acute bronchitis eye corneal opacity snakes or their isolated bladders are were... Slightly yellow, scale marks rectangular, imbricate on Crossref citations.Articles with the primers used for epilepsy and paralysis colorimetric! Can 10, 18 ( 2015 ), DNA fingerprint identification Technology, which is basionym: Zaocys Rep.,. ( applied Biosystems ) been identified, and Dinodon rufozonatum both directions with the primers used PCR... And convulsions any actions Name ( pinyin ): Shetui the dried body Zaocys.

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